Asthma biology S18 ACTIVATION OF NOCICEPTIN ORPHANIN FQ (N/OFQ) – N/OFQ PEPTIDE (NOP) RECEPTOR SYSTEM PLAYS A KEY IMMUNOMODULATORY ROLE IN ASTHMA

S18 Figure 1. N/OFQ inhibits chemoattractant-induced migration of lung mast cells and eosinophils. Migration of HMC-l cells (through an 8mm pore-size transwell membrane) was enhanced following stimulation With SCF (p<0.01, n = 5), CXCL10 (p<0.001, n = 5) and TNF-a stimulated ASM s/n (p<0.001, n = 8) and this was significantly inhibited by N/OFQ (p<0.01). HLMC migration induced by SCF (p<0.001, n = 7) was significantly attenuated by N/OFQ (p<0.001, n = 7). Migration of peripheral blood eosinophils (n = 6) stimulated by epithelial conditioned media (p<0.01) was significantly inhibited by N/OFQ (p<0.01). Cell counts were performed by a blinded observer and data was represented as fold change over control in log (2) scale (mean ± SEM). Statistical analysis was performed by one-Way ANOVA With Bonferroni’s multiple comparison test. (*: p value vs control; #: p value vs different relevant stimulation). Spoken sessions A12 Thorax 2013;68(Suppl 3):A1–A220 group.bmj.com on September 7, 2017 Published by http://thorax.bmj.com/ Downloaded from